| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.124ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This IRS1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Irs1 |
| Gene ID: |
16367 |
| Uniprot ID: |
IRS1_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in osteoblasts, but not in osteoclasts. |
| Post Translational Modifications | Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-891 is required for GRB2-binding. Phosphorylated by ALK. Phosphorylated at Ser-265, Ser-302, Ser-632 and Ser-1097 by RPS6KB1.phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-608 by TNS2 under anabolic conditions.dephosphorylation results in the proteasomal degradation of IRS1. Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). |
| Function | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. |
| Protein Name | Insulin Receptor Substrate 1Irs-1 |
| Database Links | Reactome: R-MMU-109704Reactome: -MMU-112399Reactome: -MMU-112412Reactome: -MMU-1257604Reactome: -MMU-1266695Reactome: -MMU-198203Reactome: -MMU-201556Reactome: -MMU-2428928Reactome: -MMU-5673001Reactome: -MMU-6811558Reactome: -MMU-74713Reactome: -MMU-74749 |
| Cellular Localisation | |
| Alternative ELISA Names | Insulin Receptor Substrate 1 ELISA kitIrs-1 ELISA kitIrs1 ELISA kitIrs-1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
