| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This Il2ra Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Il2ra |
| Gene ID: |
16184 |
| Uniprot ID: |
IL2RA_MOUSE |
| Specificity: |
This kit recognizes Mouse IL-2R Alpha/CD25 in samples. No significant cross-reactivity or interference between Mouse IL-2R Alpha/CD25 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | Interleukin-2 Receptor Subunit AlphaIl-2 Receptor Subunit AlphaIl-2-RaIl-2r Subunit AlphaIl2-RaP55Cd Antigen Cd25 |
| Database Links | Reactome: R-MMU-5673001Reactome: -MMU-9020558Reactome: -MMU-912526 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Interleukin-2 Receptor Subunit Alpha ELISA kitIl-2 Receptor Subunit Alpha ELISA kitIl-2-Ra ELISA kitIl-2r Subunit Alpha ELISA kitIl2-Ra ELISA kitP55 ELISA kitCd Antigen Cd25 ELISA kitIl2ra ELISA kitIl2r ELISA kit |
| Specificity | This kit recognizes Mouse Interleukin-2 Receptor alpha chain in samples. No significant cross-reactivity or interference between Mouse Interleukin-2 Receptor alpha chain and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 92 |
| EDTA plasma (n=8) | 94-109 | 99 |
| Cell culture media (n=8) | 86-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 246.98 | 579.84 | 2015.75 | 247.76 | 539.70 | 1990.30 |
| Standard deviation | 17.02 | 25.86 | 95.55 | 14.27 | 30.82 | 103.50 |
| CV (%) | 6.89 | 4.46 | 4.74 | 5.76 | 5.71 | 5.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
