| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
15.75 pg/mL |
| Detection Limit: |
31.25-2000 pg/mL |
| Short Description: |
This IL-1ra/IL-1F3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Il1rn |
| Gene ID: |
16181 |
| Uniprot ID: |
IL1RA_MOUSE |
| Specificity: |
This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Function | Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens. |
| Protein Name | Interleukin-1 Receptor Antagonist ProteinIl-1rnIl-1raIrapIl1 Inhibitor |
| Database Links | Reactome: R-MMU-9020702 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cytoplasm |
| Alternative ELISA Names | Interleukin-1 Receptor Antagonist Protein ELISA kitIl-1rn ELISA kitIl-1ra ELISA kitIrap ELISA kitIl1 Inhibitor ELISA kitIl1rn ELISA kitIl-1ra ELISA kit |
| Specificity | This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-104 | 97 |
| EDTA plasma (n=8) | 92-109 | 99 |
| Cell culture media (n=8) | 95-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.81 | 298.31 | 706.86 | 106.60 | 270.20 | 704.32 |
| Standard deviation | 5.46 | 17.78 | 21.84 | 6.12 | 11.65 | 29.58 |
| CV (%) | 5.06 | 5.96 | 3.09 | 5.74 | 4.31 | 4.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
