Mouse IL-18 (Interleukin 18) Sandwich ELISA (STJE0019964)

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STJE0019964

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 5.88 pg/mL
Detection Limit: 12.5-800 pg/mL
Short Description: This IL-18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Il18
Gene ID: 16173
Uniprot ID: IL18_MOUSE
Specificity: This kit recognizes Mouse IL-18 in samples.No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form.
Function Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Protein Name Interleukin-18
Il-18
Interferon Gamma-Inducing Factor
Ifn-Gamma-Inducing Factor
Interleukin-1 Gamma
Il-1 Gamma
Cellular Localisation Cytoplasm
Secreted
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative ELISA Names Interleukin-18 ELISA kit
Il-18 ELISA kit
Interferon Gamma-Inducing Factor ELISA kit
Ifn-Gamma-Inducing Factor ELISA kit
Interleukin-1 Gamma ELISA kit
Il-1 Gamma ELISA kit
Il18 ELISA kit
Igif ELISA kit
Specificity This kit recognizes Mouse IL-18 in samples. No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-99 90
EDTA plasma (n=8) 94-107 100
Cell culture media (n=8) 88-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 39.70 114.04 271.73 35.93 116.38 286.16
Standard deviation 2.55 6.55 11.98 2.11 5.43 14.79
CV (%) 6.42 5.74 4.41 5.88 4.67 5.17
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance