| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.21 ng/mL |
| Detection Limit: |
7.81-500 ng/mL |
| Short Description: |
This IgM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IghmUniprot ID=IGHM_MOUSE" |
| Specificity: |
This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Mu' chain is expressed in bone marrow. |
| Post Translational Modifications | Cleaved by a non-enzymatic process after Asn-96, yielding a glycosylated protein of 55 kDa. The process is induced by other proteins, and requires neutral or alkaline pH. |
| Function | IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. |
| Protein Name | Immunoglobulin Heavy Constant Mu Cleaved Into - Mu' Chain55 Kda Mu' Chain |
| Cellular Localisation | Isoform 1: SecretedDuring DifferentiationB-Lymphocytes Switch From Expression Of Membrane-Bound Igm To Secretion Of IgmIsoform 2: Cell MembraneSingle-Pass Membrane Protein |
| Alternative ELISA Names | Immunoglobulin Heavy Constant Mu Cleaved Into - Mu' Chain ELISA kit55 Kda Mu' Chain ELISA kitIghm ELISA kitIgh-6 ELISA kit |
| Specificity | This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-104 | 99 |
| EDTA plasma (n=8) | 90-101 | 95 |
| Cell culture media (n=8) | 86-96 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 23.19 | 55.70 | 206.04 | 24.07 | 60.67 | 191.96 |
| Standard deviation | 1.23 | 2.47 | 8.84 | 1.68 | 2.82 | 9.66 |
| CV (%) | 5.32 | 4.44 | 4.29 | 6.98 | 4.64 | 5.03 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
