Mouse IgM (Immunoglobulin M) Sandwich ELISA (STJE0019973)

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STJE0019973

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.21 ng/mL
Detection Limit: 7.81-500 ng/mL
Short Description: This IgM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IghmUniprot ID=IGHM_MOUSE"
Specificity: This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Mu' chain is expressed in bone marrow.
Post Translational Modifications Cleaved by a non-enzymatic process after Asn-96, yielding a glycosylated protein of 55 kDa. The process is induced by other proteins, and requires neutral or alkaline pH.
Function IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions.
Protein Name Immunoglobulin Heavy Constant Mu Cleaved Into - Mu' Chain
55 Kda Mu' Chain
Cellular Localisation Isoform 1: Secreted
During Differentiation
B-Lymphocytes Switch From Expression Of Membrane-Bound Igm To Secretion Of Igm
Isoform 2: Cell Membrane
Single-Pass Membrane Protein
Alternative ELISA Names Immunoglobulin Heavy Constant Mu Cleaved Into - Mu' Chain ELISA kit
55 Kda Mu' Chain ELISA kit
Ighm ELISA kit
Igh-6 ELISA kit
Specificity This kit recognizes Mouse IgM in samples. No significant cross-reactivity or interference between Mouse IgM and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 99
EDTA plasma (n=8) 90-101 95
Cell culture media (n=8) 86-96 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 23.19 55.70 206.04 24.07 60.67 191.96
Standard deviation 1.23 2.47 8.84 1.68 2.82 9.66
CV (%) 5.32 4.44 4.29 6.98 4.64 5.03