• Mouse IgG2a (Immunoglobulin G2a) Sandwich ELISA (STJE0004716)

Mouse IgG2a (Immunoglobulin G2a) Sandwich ELISA (STJE0004716)

SKU:
STJE0004716

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69ng/mL
Detection Limit: 7.81~500ng/mL
Short Description: This IgG2a Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Specificity: This kit recognizes Mouse IgG2a in samples. No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Specificity This kit recognizes Mouse IgG2a in samples. No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma (n=8) 93-104 99
Cell culture media (n=8) 91-108 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 26.93 53.22 216.02 27.87 56.33 215.11
Standard deviation 1.79 3.11 6.85 1.82 3.05 10.13
CV (%) 6.65 5.84 3.17 6.53 5.41 4.71
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance