| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09 ng/mL |
| Detection Limit: |
0.16-10 ng/mL |
| Short Description: |
This IGFBP-2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Igfbp2 |
| Gene ID: |
16008 |
| Uniprot ID: |
IBP2_MOUSE |
| Specificity: |
This kit recognizes Mouse IGFBP-2 in samples. No significant cross-reactivity or interference between Mouse IGFBP-2 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. |
| Post Translational Modifications | Cleaved by MMP9 leading to release of free IGF2 from IGFBP2-IGF2 complex, which contributes to enhance the motility and the growth of astrocytes. O-glycosylated. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation. Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 2Ibp-2Igf-Binding Protein 2Igfbp-2Migfbp-2 |
| Database Links | Reactome: R-MMU-381426 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor-Binding Protein 2 ELISA kitIbp-2 ELISA kitIgf-Binding Protein 2 ELISA kitIgfbp-2 ELISA kitMigfbp-2 ELISA kitIgfbp2 ELISA kitIgfbp-2 ELISA kit |
| Specificity | This kit recognizes Mouse IGFBP-2 in samples. No significant cross-reactivity or interference between Mouse IGFBP-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 96 |
| EDTA plasma (n=8) | 92-107 | 99 |
| Cell culture media (n=8) | 93-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.55 | 0.84 | 4.59 | 0.55 | 0.80 | 4.97 |
| Standard deviation | 0.03 | 0.04 | 0.18 | 0.04 | 0.04 | 0.25 |
| CV (%) | 5.75 | 4.71 | 4.02 | 6.63 | 4.53 | 5.02 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
