| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47pg/mL |
| Detection Limit: |
0.78~50pg/mL |
| Short Description: |
This IGF-2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Igf2 |
| Gene ID: |
16002 |
| Uniprot ID: |
IGF2_MOUSE |
| Specificity: |
This kit recognizes Mouse IGF-2 in samples. No significant cross-reactivity or interference between Mouse IGF-2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the heart, blood serum, kidney and skeletal muscle including the tibialis anterior muscle. |
| Post Translational Modifications | Proteolytically processed by PCSK4, proIGF2 is cleaved at Arg-128 and Arg-92 to generate big-IGF2 and mature IGF2. |
| Function | The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development (Probable). IGF2 is influenced by placental lactogen (Probable). Also involved in tissue differentiation (Probable). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable). Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. |
| Protein Name | Insulin-Like Growth Factor IiIgf-IiMultiplication-Stimulating Polypeptide Cleaved Into - Insulin-Like Growth Factor Ii - Preptin |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-2404192Reactome: -MMU-2428928Reactome: -MMU-2428933Reactome: -MMU-381426 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor Ii ELISA kitIgf-Ii ELISA kitMultiplication-Stimulating Polypeptide Cleaved Into - Insulin-Like Growth Factor Ii - Preptin ELISA kitIgf2 ELISA kitIgf-2 ELISA kit |
| Specificity | This kit recognizes Mouse IGF-2 in samples. No significant cross-reactivity or interference between Mouse IGF-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-103 | 95 |
| EDTA plasma (n=8) | 86-97 | 91 |
| Cell culture media (n=8) | 97-108 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 2.63 | 6.88 | 23.44 | 2.55 | 6.70 | 24.00 |
| Standard deviation | 0.18 | 0.32 | 1.09 | 0.14 | 0.32 | 0.78 |
| CV (%) | 6.93 | 4.58 | 4.66 | 5.68 | 4.80 | 3.27 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
