Mouse IFN-Gamma R1 (Interferon Gamma Receptor 1) Sandwich ELISA (STJE0019737)

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STJE0019737

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16-10pg/mL
Short Description: This IFN-Gamma R1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ifngr1
Gene ID: 15979
Uniprot ID: INGR1_MOUSE
Specificity: This kit recognizes Mouse IFN-Gamma R1 in samples. No significant cross-reactivity or interference between Mouse IFN-Gamma R1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed:20926559, PubMed:27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription. STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1.
Protein Name Interferon Gamma Receptor 1
Ifn-Gamma Receptor 1
Ifn-Gamma-R1
Interferon Gamma Receptor Alpha-Chain
Ifn-Gamma-R-Alpha
Cd Antigen Cd119
Database Links Reactome: R-MMU-877300
Reactome: -MMU-877312
Reactome: -MMU-9732724
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Interferon Gamma Receptor 1 ELISA kit
Ifn-Gamma Receptor 1 ELISA kit
Ifn-Gamma-R1 ELISA kit
Interferon Gamma Receptor Alpha-Chain ELISA kit
Ifn-Gamma-R-Alpha ELISA kit
Cd Antigen Cd119 ELISA kit
Ifngr1 ELISA kit
Ifngr ELISA kit
Specificity This kit recognizes Mouse IFN-γ R1 in samples. No significant cross-reactivity or interference between Mouse IFN-γ R1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 98
EDTA plasma (n=8) 87-100 92
Cell culture media (n=8) 84-95 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.02 3.38 0.51 1.07 3.47
Standard deviation 0.03 0.06 0.10 0.03 0.04 0.16
CV (%) 5.82 5.41 3.01 6.18 4.04 4.72
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance