| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.61ng/mL |
| Detection Limit: |
1.56-100ng/mL |
| Short Description: |
This IDO Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Ido1 |
| Gene ID: |
15930 |
| Uniprot ID: |
I23O1_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Highly expressed in epididymis, duodemum, jejunum, ileum, colon and spleen. Highly expressed in epididymis, prostate, duodemum, jejunum, ileum, colon and spleen, not detected in the liver (at protein level). Expressed in tumors only upon exposure to IFN gamma. Constitutively expressed in placenta in trophoblast cells. Expression is restricted to perinuclear regions of primary trophoblast giant cells (TGCs) of fetal origin at mid-gestation (10.5 dpc). After placentation (14 dpc), no IDO expression was detected at the maternal-fetal interface. |
| Post Translational Modifications | |
| Function | Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection. |
| Protein Name | Indoleamine 2 -3-Dioxygenase 1Ido-1Indoleamine-Pyrrole 2 -3-Dioxygenase |
| Database Links | Reactome: R-MMU-71240 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative ELISA Names | Indoleamine 2 -3-Dioxygenase 1 ELISA kitIdo-1 ELISA kitIndoleamine-Pyrrole 2 -3-Dioxygenase ELISA kitIdo1 ELISA kitIdo ELISA kitIndo ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
