| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Hsp90aa1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Hsp90aa1 |
| Gene ID: |
15519 |
| Uniprot ID: |
HS90A_MOUSE |
| Specificity: |
This kit recognizes Mouse HSP-90 in samples. No significant cross-reactivity or interference between Mouse HSP-90 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochondrial outer membrane which promotes host antiviral response. |
| Protein Name | Heat Shock Protein Hsp 90-AlphaHeat Shock 86 KdaHsp 86Hsp86Tumor-Specific Transplantation 86 Kda AntigenTsta |
| Database Links | Reactome: R-MMU-1227986Reactome: -MMU-1474151Reactome: -MMU-168928Reactome: -MMU-2029482Reactome: -MMU-203615Reactome: -MMU-2565942Reactome: -MMU-3371497Reactome: -MMU-3371511Reactome: -MMU-3371568Reactome: -MMU-3371571Reactome: -MMU-380259Reactome: -MMU-380270Reactome: -MMU-380284Reactome: -MMU-380320Reactome: -MMU-399954Reactome: -MMU-4420097Reactome: -MMU-5218920Reactome: -MMU-5620912Reactome: -MMU-5675482Reactome: -MMU-6798695Reactome: -MMU-8852276Reactome: -MMU-8854518Reactome: -MMU-8863795Reactome: -MMU-8939211Reactome: -MMU-9009391Reactome: -MMU-9013418Reactome: -MMU-9018519Reactome: -MMU-9652282 |
| Cellular Localisation | NucleusCytoplasmMelanosomeCell MembraneMitochondrion |
| Alternative ELISA Names | Heat Shock Protein Hsp 90-Alpha ELISA kitHeat Shock 86 Kda ELISA kitHsp 86 ELISA kitHsp86 ELISA kitTumor-Specific Transplantation 86 Kda Antigen ELISA kitTsta ELISA kitHsp90aa1 ELISA kitHsp86 ELISA kitHsp86-1 ELISA kitHspca ELISA kit |
| Specificity | This kit recognizes Mouse HSP-90 in samples. No significant cross-reactivity or interference between Mouse HSP-90 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-108 | 98 |
| EDTA plasma (n=8) | 91-103 | 97 |
| Cell culture media (n=8) | 87-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.00 | 4.80 | 0.50 | 0.90 | 4.40 |
| Standard deviation | 0.03 | 0.04 | 0.25 | 0.03 | 0.04 | 0.22 |
| CV (%) | 6.00 | 4.00 | 5.21 | 6.00 | 4.44 | 5.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
