| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
23.44pg/mL |
| Detection Limit: |
39.06~2500pg/mL |
| Short Description: |
This HSP-70/HSPA9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Hspa9 |
| Gene ID: |
15526 |
| Uniprot ID: |
GRP75_MOUSE |
| Specificity: |
This kit recognizes Mouse HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Mouse HSP-70/HSPA9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging. |
| Protein Name | Stress-70 Protein - Mitochondrial75 Kda Glucose-Regulated ProteinGrp-75Heat Shock 70 Kda Protein 9MortalinPeptide-Binding Protein 74Pbp74P66 Mot |
| Database Links | Reactome: R-MMU-3371453 |
| Cellular Localisation | MitochondrionNucleusNucleolus |
| Alternative ELISA Names | Stress-70 Protein - Mitochondrial ELISA kit75 Kda Glucose-Regulated Protein ELISA kitGrp-75 ELISA kitHeat Shock 70 Kda Protein 9 ELISA kitMortalin ELISA kitPeptide-Binding Protein 74 ELISA kitPbp74 ELISA kitP66 Mot ELISA kitHspa9 ELISA kitGrp75 ELISA kitHsp74 ELISA kitHspa9a ELISA kit |
| Specificity | This kit recognizes Mouse HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Mouse HSP-70/HSPA9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-105 | 99 |
| EDTA plasma (n=8) | 97-111 | 102 |
| Cell culture media (n=8) | 88-104 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 137.20 | 373.70 | 1104.60 | 136.00 | 354.10 | 1163.50 |
| Standard deviation | 8.80 | 18.30 | 53.00 | 9.00 | 19.80 | 37.20 |
| CV (%) | 6.41 | 4.90 | 4.80 | 6.62 | 5.59 | 3.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
