| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This mouse HPA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Hpse |
| Gene ID: |
15442 |
| Uniprot ID: |
HPSE_MOUSE |
| Specificity: |
This kit recognizes Mouse HPA in samples. No significant cross-reactivity or interference between Mouse HPA and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in skin, mainly in the stratum granulosum and the first layer of the stratum corneum in the upper part of the epidermis. Also detected in hair follicles and in sebaceous glands. |
| Post Translational Modifications | Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and the 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme. N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility. |
| Function | Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. |
| Protein Name | HeparanaseEndo-Glucoronidase Cleaved Into - Heparanase 8 Kda Subunit - Heparanase 50 Kda Subunit |
| Database Links | Reactome: R-MMU-2024096Reactome: -MMU-6798695 |
| Cellular Localisation | Lysosome MembranePeripheral Membrane ProteinSecretedNucleusProheparanase Is Secreted Via Vesicles Of The GolgiInteracts With Cell Membrane Heparan Sulfate Proteoglycans (Hspgs)Endocytosed And Accumulates In EndosomesTransferred To Lysosomes Where It Is Proteolytically Cleaved To Produce The Active EnzymeUnder Certain StimuliTransferred To The Cell SurfaceColocalizes With Sdc1 In Endosomal/Lysosomal VesiclesAccumulates In Perinuclear Lysosomal VesiclesHeparin Retains Proheparanase In The Extracellular MediumAssociates With Lipid Rafts |
| Alternative CLIA Names | Heparanase CLIA kitEndo-Glucoronidase Cleaved Into - Heparanase 8 Kda Subunit - Heparanase 50 Kda Subunit CLIA kitHpse CLIA kitHpa CLIA kit |
| Specificity | This kit recognizes Mouse HPA in samples. No significant cross-reactivity or interference between Mouse HPA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma(n=8) | 86-98 | 91 |
| Cell culture media(n=8) | 94-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 52.09 | 158.32 | 349.83 | 52.96 | 153.99 | 352.06 |
| Standard deviation | 6.17 | 13.66 | 23.09 | 6.42 | 17.49 | 23.41 |
| CV (%) | 11.84 | 8.63 | 6.60 | 12.12 | 11.36 | 6.65 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
