• Mouse HB-EGF (Heparin-binding Epidermal Growth Factor-like Growth Factor) CLIA Kit (STJC0001298)

Mouse HB-EGF (Heparin-binding Epidermal Growth Factor-like Growth Factor) CLIA Kit (STJC0001298)

SKU:
STJC0001298

£238.50 - £477.50
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Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This mouse HB-EGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Hbegf
Gene ID: 15200
Uniprot ID: HBEGF_MOUSE
Specificity: This kit recognizes Mouse HB-EGF in samples. No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Most abundant in kidney, skeletal muscle, lung, spleen, brain and heart.
Post Translational Modifications O-glycosylated.
Function Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Protein Name Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor
Hb-Egf
Hbegf
Database Links Reactome: R-MMU-1227986
Reactome: -MMU-1236394
Reactome: -MMU-1250196
Reactome: -MMU-1250342
Reactome: -MMU-1250347
Reactome: -MMU-1257604
Reactome: -MMU-177929
Reactome: -MMU-179812
Reactome: -MMU-180292
Reactome: -MMU-180336
Reactome: -MMU-182971
Reactome: -MMU-1963640
Reactome: -MMU-1963642
Reactome: -MMU-212718
Reactome: -MMU-2179392
Reactome: -MMU-5673001
Reactome: -MMU-6785631
Reactome: -MMU-6811558
Reactome: -MMU-8847993
Reactome: -MMU-8856825
Reactome: -MMU-8856828
Reactome: -MMU-8857538
Reactome: -MMU-8863795
Reactome: -MMU-9009391
Cellular Localisation Heparin-Binding Egf-Like Growth Factor: Secreted
Extracellular Space
Mature Hb-Egf Is Released Into The Extracellular Space And Probably Binds To A Receptor
Proheparin-Binding Egf-Like Growth Factor: Cell Membrane
Single-Pass Type I Membrane Protein
Alternative CLIA Names Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor CLIA kit
Hb-Egf CLIA kit
Hbegf CLIA kit
Hbegf CLIA kit
Dtr CLIA kit
Hegfl CLIA kit
Specificity This kit recognizes Mouse HB-EGF in samples. No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 102-114 108
EDTA plasma(n=8) 100-115 106
Cell culture media(n=8) 94-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 26.55 43.56 186.52 26.51 46.06 197.33
Standard deviation 2.56 3.86 20.42 2.90 4.09 20.76
CV (%) 9.64 8.86 10.95 10.94 8.88 10.52
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance