| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This mouse GzmsA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Gzma |
| Gene ID: |
14938 |
| Uniprot ID: |
GRAA_MOUSE |
| Specificity: |
This kit recognizes Mouse GzmsA in samples. No significant cross-reactivity or interference between Mouse GzmsA and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Found in cytotoxic lymphocytes and in normal lymphoid tissues such as thymus and spleen. Isoform HF1: More abundant in lymphoid tissues than isoform HF2. |
| Function | Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. |
| Protein Name | Granzyme AAutocrine Thymic Lymphoma Granzyme-Like Serine ProteaseCtla-3Fragmentin-1T Cell-Specific Serine Protease 1Tsp-1 |
| Cellular Localisation | SecretedCytoplasmic GranuleDelivered Into The Target Cell By Perforin |
| Alternative CLIA Names | Granzyme A CLIA kitAutocrine Thymic Lymphoma Granzyme-Like Serine Protease CLIA kitCtla-3 CLIA kitFragmentin-1 CLIA kitT Cell-Specific Serine Protease 1 CLIA kitTsp-1 CLIA kitGzma CLIA kitCtla-3 CLIA kitCtla3 CLIA kitMtsp-1 CLIA kit |
| Specificity | This kit recognizes Mouse GzmA in samples. No significant cross-reactivity or interference between Mouse GzmA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-114 | 104 |
| EDTA plasma(n=8) | 90-102 | 96 |
| Cell culture media(n=8) | 96-113 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 96.99 | 219.05 | 896.56 | 91.93 | 201.33 | 938.64 |
| Standard deviation | 9.33 | 22.83 | 79.79 | 9.12 | 22.91 | 102.97 |
| CV (%) | 9.62 | 10.42 | 8.90 | 9.92 | 11.38 | 10.97 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
