Applications: | ELISA |
Reactivity: | Mouse |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description: | This Gzmb Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | Gzmb |
Gene ID: | 14939 |
Uniprot ID: | GRAB_MOUSE |
Specificity: | This kit recognizes Mouse GzmB in samples. No significant cross-reactivity or interference between Mouse GzmB and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Protein Name | Granzyme B(G -H Ctla-1 Cytotoxic Cell Protease 1 Ccp1 Fragmentin-2 |
Database Links | Reactome: R-MMU-5620971 Reactome: -MMU-75108 |
Cellular Localisation | Secreted Cytolytic Granule Delivered Into The Target Cell By Perforin |
Alternative ELISA Names | Granzyme B(G -H ELISA kit Ctla-1 ELISA kit Cytotoxic Cell Protease 1 ELISA kit Ccp1 ELISA kit Fragmentin-2 ELISA kit Gzmb ELISA kit Ctla-1 ELISA kit Ctla1 ELISA kit |
Specificity | This kit recognizes Mouse GzmB in samples. No significant cross-reactivity or interference between Mouse GzmB and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 85-96 | 91 |
EDTA plasma (n=8) | 94-108 | 100 |
Cell culture media (n=8) | 93-108 | 101 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 50.92 | 155.11 | 363.59 | 51.09 | 158.50 | 378.21 |
Standard deviation | 3.56 | 9.10 | 16.33 | 3.43 | 7.04 | 16.79 |
CV (%) | 6.99 | 5.87 | 4.49 | 6.71 | 4.44 | 4.44 |