| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
10.01pg/mL |
| Detection Limit: |
15.63-1000pg/mL |
| Short Description: |
This GRO Alpha/CXCL1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Cxcl1 |
| Gene ID: |
14825 |
| Uniprot ID: |
GROA_MOUSE |
| Specificity: |
This kit recognizes Mouse GRO Alpha/CXCL1 in samples. No significant cross-reactivity or interference between Mouse GRO Alpha/CXCL1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity. |
| Protein Name | Growth-Regulated Alpha ProteinC-X-C Motif Chemokine 1Platelet-Derived Growth Factor-Inducible Protein KcSecretory Protein N51 Cleaved Into - Kc(5-72Hematopoietic Synergistic FactorHsfKc-T |
| Database Links | Reactome: R-MMU-380108Reactome: -MMU-418594Reactome: -MMU-6798695 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Growth-Regulated Alpha Protein ELISA kitC-X-C Motif Chemokine 1 ELISA kitPlatelet-Derived Growth Factor-Inducible Protein Kc ELISA kitSecretory Protein N51 Cleaved Into - Kc(5-72 ELISA kitHematopoietic Synergistic Factor ELISA kitHsf ELISA kitKc-T ELISA kitCxcl1 ELISA kitGro ELISA kitGro1 ELISA kitMgsa ELISA kitScyb1 ELISA kit |
| Specificity | This kit recognizes Mouse GROα/CXCL1 in samples. No significant cross-reactivity or interference between Mouse GROα/CXCL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-108 | 100 |
| EDTA plasma (n=8) | 85-98 | 92 |
| Cell culture media (n=8) | 94-106 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 49.96 | 139.61 | 359.77 | 47.33 | 130.77 | 359.19 |
| Standard deviation | 2.86 | 7.86 | 17.34 | 2.62 | 5.30 | 12.72 |
| CV (%) | 5.72 | 5.63 | 4.82 | 5.53 | 4.05 | 3.54 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
