| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This Grn Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Grn |
| Gene ID: |
14824 |
| Uniprot ID: |
GRN_MOUSE |
| Specificity: |
This kit recognizes Mouse GRN in samples. No significant cross-reactivity or interference between Mouse GRN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome. |
| Function | Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. |
| Protein Name | ProgranulinPgrnAcrograninEpithelin/Granulin PrecursorGlycoprotein Of 88 KdaGp88Glycoprotein 88Pc Cell-Derived Growth FactorPcdgfProepithelinPepi Cleaved Into - Paragranulin - Granulin-1 - Granulin-2 - Granulin-3 - Granulin-4 - Granulin-5 - Granulin-6 - Granulin-7 |
| Database Links | Reactome: R-MMU-6798695 |
| Cellular Localisation | SecretedLysosomeEndocytosed By Sort1 And Delivred To LysosomesTargeted To Lysosome By Psap Via M6pr And Lrp1In Both Biosynthetic And Endocytic PathwaysCo-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome |
| Alternative ELISA Names | Progranulin ELISA kitPgrn ELISA kitAcrogranin ELISA kitEpithelin/Granulin Precursor ELISA kitGlycoprotein Of 88 Kda ELISA kitGp88 ELISA kitGlycoprotein 88 ELISA kitPc Cell-Derived Growth Factor ELISA kitPcdgf ELISA kitProepithelin ELISA kitPepi Cleaved Into - Paragranulin - Granulin-1 - Granulin-2 - Granulin-3 - Granulin-4 - Granulin-5 - Granulin-6 - Granulin-7 ELISA kitGrn ELISA kit |
| Specificity | This kit recognizes Mouse GRN in samples. No significant cross-reactivity or interference between Mouse GRN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-98 | 93 |
| EDTA plasma (n=8) | 90-103 | 97 |
| Cell culture media (n=8) | 84-98 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.57 | 9.33 | 47.17 | 4.77 | 10.18 | 48.22 |
| Standard deviation | 0.24 | 0.46 | 2.02 | 0.26 | 0.50 | 2.50 |
| CV (%) | 5.25 | 4.93 | 4.28 | 5.45 | 4.91 | 5.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
