• Mouse Ghrl (Appetite-regulating hormone) Competitive ELISA (STJE0004603)

Mouse Ghrl (Appetite-regulating hormone) Competitive ELISA (STJE0004603)

SKU:
STJE0004603

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Ghrl Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: Ghrl
Gene ID: 58991
Uniprot ID: GHRL_MOUSE
Specificity: This kit recognizes Mouse GHRL in samples. No significant cross-reactivity or interference between Mouse GHRL and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-octanoylated by GOAT/MBOAT4. O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated form ghrelin-C10 differs in the length of the carbon backbone of the carboxylic acid bound to Ser-26. Amidation of Leu-98 is essential for obestatin activity.
Function Ghrelin: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin: Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
Protein Name Appetite-Regulating Hormone
Growth Hormone Secretagogue
Growth Hormone-Releasing Peptide
Motilin-Related Peptide
Protein M46 Cleaved Into - Ghrelin - Obestatin
Database Links Reactome: R-MMU-416476
Reactome: -MMU-422085
Cellular Localisation Secreted
Alternative ELISA Names Appetite-Regulating Hormone ELISA kit
Growth Hormone Secretagogue ELISA kit
Growth Hormone-Releasing Peptide ELISA kit
Motilin-Related Peptide ELISA kit
Protein M46 Cleaved Into - Ghrelin - Obestatin ELISA kit
Ghrl ELISA kit
Mtlrp ELISA kit
Specificity This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 97
EDTA plasma(n=8) 90-107 97
Cell culture media(n=8) 87-99 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.20 3.80 0.50 1.30 3.50
Standard deviation 0.03 0.05 0.13 0.03 0.05 0.16
CV (%) 6.00 4.17 3.42 6.00 3.85 4.57
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance