| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This GHRL Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GHRL |
| Gene ID: |
51738 |
| Uniprot ID: |
GHRL_HUMAN |
| Specificity: |
This kit recognizes Human GHRL in samples. No significant cross-reactivity or interference between Human GHRL and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | O-octanoylated by GOAT/MBOAT4. O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid. Also O-acetylated and O-butyrylated on Ser-26 to minor levels. Amidation of Leu-98 is essential for obestatin activity. |
| Function | Ghrelin-27: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Ghrelin-28: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin: May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. |
| Protein Name | Appetite-Regulating HormoneGrowth Hormone SecretagogueGrowth Hormone-Releasing PeptideMotilin-Related PeptideProtein M46 Cleaved Into - Ghrelin-27 - Ghrelin-28Ghrelin - Obestatin |
| Database Links | Reactome: R-HSA-375276 Q9UBU3-1Reactome: R-HSA-416476 Q9UBU3-1Reactome: R-HSA-422085 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Appetite-Regulating Hormone ELISA kitGrowth Hormone Secretagogue ELISA kitGrowth Hormone-Releasing Peptide ELISA kitMotilin-Related Peptide ELISA kitProtein M46 Cleaved Into - Ghrelin-27 - Ghrelin-28 ELISA kitGhrelin - Obestatin ELISA kitGHRL ELISA kitMTLRP ELISA kitUNQ524 ELISA kitPRO1066 ELISA kit |
| Specificity | This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-97 | 92 |
| EDTA plasma(n=8) | 95-108 | 101 |
| Cell culture media(n=8) | 91-105 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 0.90 | 4.10 | 0.50 | 0.90 | 3.70 |
| Standard deviation | 0.03 | 0.04 | 0.13 | 0.03 | 0.04 | 0.13 |
| CV (%) | 6.00 | 4.44 | 3.17 | 6.00 | 4.44 | 3.51 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
