| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This mouse Gas6 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Gas6 |
| Gene ID: |
14456 |
| Uniprot ID: |
GAS6_MOUSE |
| Specificity: |
This kit recognizes Mouse Gas6 in samples. No significant cross-reactivity or interference between Mouse Gas6 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. |
| Function | Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. |
| Protein Name | Growth Arrest-Specific Protein 6Gas-6Axl Receptor Tyrosine Kinase Ligand |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-159763Reactome: -MMU-159782Reactome: -MMU-202733Reactome: -MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Growth Arrest-Specific Protein 6 CLIA kitGas-6 CLIA kitAxl Receptor Tyrosine Kinase Ligand CLIA kitGas6 CLIA kit |
| Specificity | This kit recognizes Mouse Gas6 in samples. No significant cross-reactivity or interference between Mouse Gas6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma(n=8) | 86-98 | 91 |
| Cell culture media(n=8) | 89-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 46.83 | 128.30 | 499.11 | 42.52 | 137.00 | 535.43 |
| Standard deviation | 4.76 | 12.33 | 56.95 | 4.93 | 10.23 | 48.08 |
| CV (%) | 10.16 | 9.61 | 11.41 | 11.59 | 7.47 | 8.98 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
