| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This mouse FPR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Fpr2 |
| Gene ID: |
14289 |
| Uniprot ID: |
FPR2_MOUSE |
| Specificity: |
This kit recognizes Mouse FPR2 in samples. No significant cross-reactivity or interference between Mouse FPR2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Primarily expressed in neutrophils. Not detected in vomeronasal neurons. |
| Function | High affinity receptor for N-formyl-methionyl peptides (FMLP), which are powerful neutrophil chemotactic factors. Stimulates chemotaxis in immune cells to site of infection or tissue damage upon recognition of several ligands, such as FMLP, or ligand involved in cell damage, disease or inflammation. Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation. |
| Protein Name | Formyl Peptide Receptor 2Formylpeptide Receptor-Related Sequence 2Lipoxin A4 Receptor-Like ProteinN-Formylpeptide Receptor-Like 2 |
| Database Links | Reactome: R-MMU-416476Reactome: -MMU-418594Reactome: -MMU-444473Reactome: -MMU-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinAssociates With Amyloid-Beta Protein 42Product Of AppAt The Cell Surface And The Complex Is Then Rapidly Internalized |
| Alternative CLIA Names | Formyl Peptide Receptor 2 CLIA kitFormylpeptide Receptor-Related Sequence 2 CLIA kitLipoxin A4 Receptor-Like Protein CLIA kitN-Formylpeptide Receptor-Like 2 CLIA kitFpr2 CLIA kitFpr-rs2 CLIA kit |
| Specificity | This kit recognizes Mouse FPR2 in samples. No significant cross-reactivity or interference between Mouse FPR2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-101 | 92 |
| EDTA plasma(n=8) | 92-104 | 98 |
| Cell culture media(n=8) | 87-99 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 40.32 | 72.77 | 367.79 | 37.73 | 76.79 | 402.90 |
| Standard deviation | 3.92 | 6.64 | 30.05 | 4.83 | 8.35 | 26.75 |
| CV (%) | 9.72 | 9.12 | 8.17 | 12.80 | 10.87 | 6.64 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
