• Mouse FGFR3 (Fibroblast Growth Factor Receptor 3) Sandwich ELISA Kit (STJE0008919)

Mouse FGFR3 (Fibroblast Growth Factor Receptor 3) Sandwich ELISA Kit (STJE0008919)

SKU:
STJE0008919

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.057ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This FGFR3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Fgfr3Uniprot ID=FGFR3_MOUSE"
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity In embryo, expressed in heart, lung, kidney, skin, head and liver but not in muscle. In adult, highest levels in brain. Also expressed in liver, lung, kidney, testis, ovary and uterus. Very low levels in heart, thymus, spleen and muscle.
Post Translational Modifications Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-719 is essential for stimulation of cell proliferation and activation of PIK3R1, STAT1 and MAP kinase signaling. Phosphorylation at Tyr-755 is required for interaction with PIK3R1 and PLCG1. Ubiquitinated. Is rapidly ubiquitinated after ligand binding and autophosphorylation, leading to receptor internalization and degradation. Subject to both proteasomal and lysosomal degradation. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.
Function Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of STAT1, STAT5A and STAT5B. Plays a role in postnatal lung development.
Protein Name Fibroblast Growth Factor Receptor 3
Fgfr-3
Heparin-Binding Growth Factor Receptor
Cd Antigen Cd333
Database Links
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle
Endoplasmic Reticulum
The Activated Receptor Is Rapidly Internalized And Degraded
Detected In Intracellular Vesicles After Internalization Of The Autophosphorylated Receptor
Alternative ELISA Names Fibroblast Growth Factor Receptor 3 ELISA kit
Fgfr-3 ELISA kit
Heparin-Binding Growth Factor Receptor ELISA kit
Cd Antigen Cd333 ELISA kit
Fgfr3 ELISA kit
Mfr3 ELISA kit
Sam3 ELISA kit
output

Information sourced from Uniprot.org

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