| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.1ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This FAS/CD95 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Fas |
| Gene ID: |
14102 |
| Uniprot ID: |
TNR6_MOUSE |
| Specificity: |
This kit recognizes Mouse FAS/CD95 in samples. No significant crossreactivity or interference between Mouse FAS/CD95 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Detected in various tissues including thymus, liver, lung, heart, and adult ovary. |
| Post Translational Modifications | Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 6Apo-1 AntigenApoptosis-Mediating Surface Antigen FasFaslg ReceptorCd Antigen Cd95 |
| Database Links | Reactome: R-MMU-3371378Reactome: -MMU-5218900Reactome: -MMU-69416Reactome: -MMU-75157 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative ELISA Names | Tumor Necrosis Factor Receptor Superfamily Member 6 ELISA kitApo-1 Antigen ELISA kitApoptosis-Mediating Surface Antigen Fas ELISA kitFaslg Receptor ELISA kitCd Antigen Cd95 ELISA kitFas ELISA kitApt1 ELISA kitTnfrsf6 ELISA kit |
| Specificity | This kit recognizes Mouse FAS/CD95 in samples. No significant cross-reactivity or interference between Mouse FAS/CD95 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 94 |
| EDTA plasma (n=8) | 86-98 | 92 |
| Cell culture media (n=8) | 87-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 1.58 | 3.65 | 0.44 | 1.50 | 3.93 |
| Standard deviation | 0.03 | 0.06 | 0.11 | 0.03 | 0.08 | 0.17 |
| CV (%) | 5.23 | 4.04 | 3.02 | 6.89 | 5.64 | 4.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
