Mouse Fabp3 (Fatty acid-binding protein, heart) Sandwich ELISA (STJE0005169)

SKU:
STJE0005169

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Fabp3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Fabp3
Gene ID: 14077
Uniprot ID: FABPH_MOUSE
Specificity: This kit recognizes Mouse FABP3 in samples. No significant cross-reactivity or interference between Mouse FABP3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Fatty Acid-Binding Protein - Heart
Fatty Acid-Binding Protein 3
Heart-Type Fatty Acid-Binding Protein
H-Fabp
Mammary-Derived Growth Inhibitor
Mdgi
Database Links Reactome: R-MMU-163560
Cellular Localisation Cytoplasm
Alternative ELISA Names Fatty Acid-Binding Protein - Heart ELISA kit
Fatty Acid-Binding Protein 3 ELISA kit
Heart-Type Fatty Acid-Binding Protein ELISA kit
H-Fabp ELISA kit
Mammary-Derived Growth Inhibitor ELISA kit
Mdgi ELISA kit
Fabp3 ELISA kit
Fabph1 ELISA kit
Specificity This kit recognizes Mouse FABP3 in samples. No significant cross-reactivity or interference between Mouse FABP3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 95
EDTA plasma (n=8) 90-104 95
Cell culture media (n=8) 95-110 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 214.88 430.91 1826.64 200.29 418.87 1774.01
Standard deviation 11.17 22.02 74.53 12.32 19.69 71.67
CV (%) 5.20 5.11 4.08 6.15 4.70 4.04
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance