Mouse DPP4 (Dipeptidyl Peptidase 4) Sandwich ELISA (STJE0019797)

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STJE0019797

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description: This DPP4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Dpp4
Gene ID: 13482
Uniprot ID: DPP4_MOUSE
Specificity: This kit recognizes Mouse DPP4 in samples. No significant cross-reactivity or interference between Mouse DPP4 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
Function Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
Protein Name Dipeptidyl Peptidase 4
Dipeptidyl Peptidase Iv
Dpp Iv
T-Cell Activation Antigen Cd26
Thymocyte-Activating Molecule
Tham
Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form
Dipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form
Dipeptidyl Peptidase Iv Soluble Form
Database Links Reactome: R-MMU-381771
Reactome: -MMU-400511
Cellular Localisation Dipeptidyl Peptidase 4 Soluble Form: Secreted
Detected In The Serum And The Seminal Fluid
Cell Membrane
Single-Pass Type Ii Membrane Protein
Apical Cell Membrane
Cell Projection
Invadopodium Membrane
Lamellipodium Membrane
Cell Junction
Membrane Raft
Translocated To The Apical Membrane Through The Concerted Action Of N- And O-Glycans And Its Association With Lipid Microdomains Containing Cholesterol And Sphingolipids
Redistributed To Membrane Rafts In T-Cell In An Interleukin-12-Dependent Activation
Its Interaction With Cav1 Is Necessary For Its Translocation To Membrane Rafts
Colocalized With Ptprc In Membrane Rafts
Colocalized With Fap In Invadopodia And Lamellipodia Of Migratory Activated Endothelial Cells In Collagenous Matrix
Colocalized With Fap On Endothelial Cells Of Capillary-Like Microvessels But Not Large Vessels Within Invasive Breast Ductal Carcinoma
Colocalized With Ada At The Cell Junction In Lymphocyte-Epithelial Cell Adhesion
Colocalized With Igf2r In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface
Alternative ELISA Names Dipeptidyl Peptidase 4 ELISA kit
Dipeptidyl Peptidase Iv ELISA kit
Dpp Iv ELISA kit
T-Cell Activation Antigen Cd26 ELISA kit
Thymocyte-Activating Molecule ELISA kit
Tham ELISA kit
Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form ELISA kit
Dipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form ELISA kit
Dipeptidyl Peptidase Iv Soluble Form ELISA kit
Dpp4 ELISA kit
Cd26 ELISA kit
Specificity This kit recognizes Mouse DPP4 in samples. No significant cross-reactivity or interference between Mouse DPP4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 100
EDTA plasma (n=8) 96-109 102
Cell culture media (n=8) 92-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 5.08 12.83 42.40 5.42 11.68 43.18
Standard deviation 0.34 0.76 1.45 0.37 0.70 1.31
CV (%) 6.65 5.89 3.43 6.82 5.99 3.03
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance