| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.058ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This DNASE1L3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Dnase1l3 |
| Gene ID: |
13421 |
| Uniprot ID: |
DNSL3_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed at high levels in liver, spleen and testes. Expressed at lower levels in heart, lungs, skeletal muscle and kidney. Not expressed in brain. Predominantly expressed in macrophages.at protein level. Secreted by mononuclear phagocytes. |
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. ADP-ribosylation negatively regulates enzymatic activity during apoptosis. |
| Function | Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation. |
| Protein Name | Deoxyribonuclease GammaDnase GammaDnase I Homolog Protein Dhp2Deoxyribonuclease I-Like 3Dnase I-Like 3Liver And Spleen DnaseLs-DnaseLsd |
| Database Links | |
| Cellular Localisation | NucleusEndoplasmic ReticulumSecretedContradictory Reports Exist About The Subcellular Localization Under Normal Physiological ConditionsShown To Translocate To Rough Endoplasmic Reticulum And To Be Transported Through The Entire Secretory Pathway For SecretionHoweverUnder Conditions Of Cell DeathMay Diffuse And/Or Be Actively Transported To The Nucleus |
| Alternative ELISA Names | Deoxyribonuclease Gamma ELISA kitDnase Gamma ELISA kitDnase I Homolog Protein Dhp2 ELISA kitDeoxyribonuclease I-Like 3 ELISA kitDnase I-Like 3 ELISA kitLiver And Spleen Dnase ELISA kitLs-Dnase ELISA kitLsd ELISA kitDnase1l3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
