• Mouse DAPK3 (Death Associated Protein Kinase 3) Sandwich ELISA Kit (STJE0008357)

Mouse DAPK3 (Death Associated Protein Kinase 3) Sandwich ELISA Kit (STJE0008357)

SKU:
STJE0008357

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.058ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This DAPK3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Dapk3
Gene ID: 13144
Uniprot ID: DAPK3_MOUSE
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Highly expressed in heart, brain, lung, skeletal muscle, kidney and testis. Lower levels in liver and spleen.
Post Translational Modifications Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus. The phosphorylation status is critical for kinase activity, oligomerization and intracellular localization. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. The phosphorylated form is localized in the cytoplasm and nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation increases the trimeric form, and its dephosphorylation favors a kinase-inactive monomeric form.
Function Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation.in vitro phosphorylates PAWR. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2.disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition.
Protein Name Death-Associated Protein Kinase 3
Dap Kinase 3
Dap-Like Kinase
Dlk
Mypt1 Kinase
Zip-Kinase
Database Links
Cellular Localisation Nucleus
Pml Body
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Chromosome
Centromere
Predominantly Localized To The Nucleus
Relocates To The Cytoplasm On Binding Pawr Where The Complex Appears To Interact With Actin Filaments
Associated With The Centrosomes Throughout The Mitotic Cell Cycle
With The Centromeres From Prophase To Anaphase And With The Contractile Ring During Cytokinesis
Alternative ELISA Names Death-Associated Protein Kinase 3 ELISA kit
Dap Kinase 3 ELISA kit
Dap-Like Kinase ELISA kit
Dlk ELISA kit
Mypt1 Kinase ELISA kit
Zip-Kinase ELISA kit
Dapk3 ELISA kit
Zipk ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance