| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.054ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This DAG1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Dag1 |
| Gene ID: |
13138 |
| Uniprot ID: |
DAG1_MOUSE |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Detected in brain and kidney (at protein level). Detected in sciatic nerve (at protein level). Expressed in neurons and muscle cells (at protein level). Expressed in a variety of tissues. In brain, expressed in the hippocampal formation, the olfactory bulb, the cerebellum and the thalamus. In the peripheral nerve system, expressed in Schwann cells. |
| Post Translational Modifications | Alpha-dystroglycan: O-glycosylated. POMGNT1 catalyzes the initial addition of N-acetylglucosamine, giving rise to the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety and thus providing the necessary basis for the addition of further carbohydrate moieties. Heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-315, Thr-317, Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE1) protein amd is required for laminin binding. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The brain form displays a unique glycosylation pattern which is absent in other tissues.this form shows enhanced binding to laminin LAMA5 compared to the skeletal muscle form. Beta-dystroglycan: N-glycosylated. Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglycan can occur releasing a peptide of about 30 kDa. SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry. |
| Function | The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan: Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains, and for certain adenoviruses. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5. Beta-dystroglycan: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity. |
| Protein Name | Dystroglycan 1DystroglycanDystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-DystroglycanAlpha-Dg - Beta-DystroglycanBeta-Dg |
| Database Links | Reactome: R-MMU-5173105Reactome: -MMU-9010553 |
| Cellular Localisation | Alpha-Dystroglycan: SecretedExtracellular SpaceBeta-Dystroglycan: Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmCytoskeletonNucleusNucleoplasmCell MembraneSarcolemmaPostsynaptic Cell MembraneThe Monomeric Form Translocates To The Nucleus Via The Action Of Importins And Depends On RanNuclear Transport Is Inhibited By Tyr-892 PhosphorylationIn Skeletal MuscleThis Phosphorylated Form Locates To A Vesicular Internal Membrane CompartmentIn Muscle CellsSarcolemma Localization Requires The Presence Of Ank2While Localization To Costameres Requires The Presence Of Ank3Localizes To Neuromuscular Junctions (Nmjs)In Adult MuscleNmj Localization Depends Upon Ank2 PresenceBut Not In Newborn AnimalsIn Peripheral NervesLocalizes To The Schwann Cell MembraneColocalizes With Erm Proteins In Schwann-Cell Microvilli |
| Alternative ELISA Names | Dystroglycan 1 ELISA kitDystroglycan ELISA kitDystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan ELISA kitAlpha-Dg - Beta-Dystroglycan ELISA kitBeta-Dg ELISA kitDag1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
