| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.214ng/mL |
| Detection Limit: |
0.625-40ng/mL |
| Short Description: |
This CYP27B1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Cyp27b1 |
| Gene ID: |
13115 |
| Uniprot ID: |
CP27B_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Kidney. |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial25-Ohd-1 Alpha-Hydroxylase25-Hydroxyvitamin D(3 1-Alpha-HydroxylaseVd3 1a HydroxylaseCalcidiol 1-MonooxygenaseCytochrome P450 Subfamily Xxviib Polypeptide 1Cytochrome P450c1 AlphaCytochrome P450vd1-AlphaCytochrome P450 27b1 |
| Database Links | Reactome: R-MMU-196791Reactome: -MMU-211916 |
| Cellular Localisation | Mitochondrion Membrane |
| Alternative ELISA Names | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial ELISA kit25-Ohd-1 Alpha-Hydroxylase ELISA kit25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase ELISA kitVd3 1a Hydroxylase ELISA kitCalcidiol 1-Monooxygenase ELISA kitCytochrome P450 Subfamily Xxviib Polypeptide 1 ELISA kitCytochrome P450c1 Alpha ELISA kitCytochrome P450vd1-Alpha ELISA kitCytochrome P450 27b1 ELISA kitCyp27b1 ELISA kitCyp27b ELISA kitCyp40 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
