• Mouse CYP27B1 (Cytochrome P450 27B1) Sandwich ELISA Kit (STJE0008315)

Mouse CYP27B1 (Cytochrome P450 27B1) Sandwich ELISA Kit (STJE0008315)

SKU:
STJE0008315

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.214ng/mL
Detection Limit: 0.625-40ng/mL
Short Description: This CYP27B1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Cyp27b1
Gene ID: 13115
Uniprot ID: CP27B_MOUSE
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Kidney.
Post Translational Modifications
Function A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
Protein Name 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial
25-Ohd-1 Alpha-Hydroxylase
25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase
Vd3 1a Hydroxylase
Calcidiol 1-Monooxygenase
Cytochrome P450 Subfamily Xxviib Polypeptide 1
Cytochrome P450c1 Alpha
Cytochrome P450vd1-Alpha
Cytochrome P450 27b1
Database Links Reactome: R-MMU-196791
Reactome: -MMU-211916
Cellular Localisation Mitochondrion Membrane
Alternative ELISA Names 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial ELISA kit
25-Ohd-1 Alpha-Hydroxylase ELISA kit
25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase ELISA kit
Vd3 1a Hydroxylase ELISA kit
Calcidiol 1-Monooxygenase ELISA kit
Cytochrome P450 Subfamily Xxviib Polypeptide 1 ELISA kit
Cytochrome P450c1 Alpha ELISA kit
Cytochrome P450vd1-Alpha ELISA kit
Cytochrome P450 27b1 ELISA kit
Cyp27b1 ELISA kit
Cyp27b ELISA kit
Cyp40 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance