| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.060ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This CX3CL1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Cx3cl1 |
| Gene ID: |
20312 |
| Uniprot ID: |
X3CL1_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis. Highly expressed in lesional smooth muscle cells, but not macrophages. Low levels of ABCD-3 mRNA were also found in anti-CD40-stimulated splenic B-cells, but not in resting B-cells. Also expressed in dendritic cells. |
| Post Translational Modifications | A soluble short 80 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. |
| Function | Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. Processed fractalkine: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. |
| Protein Name | FractalkineFkAbcd-3C-X3-C Motif Chemokine 1Cx3c Membrane-Anchored ChemokineNeurotactinSmall-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine |
| Database Links | Reactome: R-MMU-380108Reactome: -MMU-418594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinProcessed Fractalkine: Secreted |
| Alternative ELISA Names | Fractalkine ELISA kitFk ELISA kitAbcd-3 ELISA kitC-X3-C Motif Chemokine 1 ELISA kitCx3c Membrane-Anchored Chemokine ELISA kitNeurotactin ELISA kitSmall-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine ELISA kitCx3cl1 ELISA kitCx3c ELISA kitFkn ELISA kitScyd1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
