| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This CNP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Nppc |
| Gene ID: |
18159 |
| Uniprot ID: |
ANFC_MOUSE |
| Specificity: |
This kit recognizes Mouse CNP in samples. No significant cross-reactivity or interference between Mouse CNP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | CNP-22: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Acts by specifically binding and stimulating NPR2 to produce cGMP. Binds the clearance receptor NPR3. |
| Protein Name | C-Type Natriuretic Peptide Cleaved Into - Cnp-22 - Cnp-29 - Cnp-53 |
| Database Links | Reactome: R-MMU-5578768 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-Type Natriuretic Peptide Cleaved Into - Cnp-22 - Cnp-29 - Cnp-53 ELISA kitNppc ELISA kitCnp ELISA kit |
| Specificity | This kit recognizes CNP in samples. No significant cross-reactivity or interference between CNP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 94 |
| EDTA plasma(n=8) | 94-107 | 99 |
| Cell culture media(n=8) | 93-106 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.20 | 150.10 | 345.40 | 54.10 | 162.30 | 320.10 |
| Standard deviation | 2.90 | 8.00 | 16.90 | 3.80 | 9.10 | 14.40 |
| CV (%) | 5.66 | 5.33 | 4.89 | 7.02 | 5.61 | 4.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
