| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
6.9pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This CKB Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Ckb |
| Gene ID: |
12709 |
| Uniprot ID: |
KCRB_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in hippocampus and corpus callosum (at protein level). |
| Post Translational Modifications | |
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Creatine Kinase B-TypeB-CkCreatine Kinase B ChainCreatine Phosphokinase B-TypeCpk-B |
| Database Links | Reactome: R-MMU-71288Reactome: -MMU-9696264 |
| Cellular Localisation | CytoplasmCytosolMitochondrionCell MembraneLocalizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region |
| Alternative ELISA Names | Creatine Kinase B-Type ELISA kitB-Ck ELISA kitCreatine Kinase B Chain ELISA kitCreatine Phosphokinase B-Type ELISA kitCpk-B ELISA kitCkb ELISA kitCkbb ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
