| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.72ng/mL |
| Detection Limit: |
1.56-100ng/mL |
| Short Description: |
This CD14 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Cd14 |
| Gene ID: |
12475 |
| Uniprot ID: |
CD14_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Detected on peritoneal macrophages (at protein level). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level). |
| Post Translational Modifications | |
| Function | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Acts as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with coreceptors TLR2 and TLR1. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
| Protein Name | Monocyte Differentiation Antigen Cd14Myeloid Cell-Specific Leucine-Rich GlycoproteinCd Antigen Cd14 |
| Database Links | Reactome: R-MMU-140534Reactome: -MMU-166016Reactome: -MMU-166020Reactome: -MMU-166166Reactome: -MMU-2562578Reactome: -MMU-5686938Reactome: -MMU-6798695Reactome: -MMU-936964Reactome: -MMU-937041Reactome: -MMU-937072Reactome: -MMU-975163 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedMembrane RaftGolgi ApparatusSolubleSecreted Forms Seem To ExistThey May Arise By Cleavage Of The Gpi Anchor |
| Alternative ELISA Names | Monocyte Differentiation Antigen Cd14 ELISA kitMyeloid Cell-Specific Leucine-Rich Glycoprotein ELISA kitCd Antigen Cd14 ELISA kitCd14 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
