Mouse Ccl11 (Eotaxin) Sandwich ELISA (STJE0005342)

SKU:
STJE0005342

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5ng/mL
Detection Limit: 62.5~4000ng/mL
Short Description: This Ccl11 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ccl11
Gene ID: 20292
Uniprot ID: CCL11_MOUSE
Specificity: This kit recognizes Mouse ECF/CCL11 in samples. No significant cross-reactivity or interference between Mouse ECF/CCL11 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Protein Name Eotaxin
C-C Motif Chemokine 11
Eosinophil Chemotactic Protein
Small-Inducible Cytokine A11
Database Links Reactome: R-MMU-380108
Reactome: -MMU-418594
Cellular Localisation Secreted
Alternative ELISA Names Eotaxin ELISA kit
C-C Motif Chemokine 11 ELISA kit
Eosinophil Chemotactic Protein ELISA kit
Small-Inducible Cytokine A11 ELISA kit
Ccl11 ELISA kit
Scya11 ELISA kit
Specificity This kit recognizes Mouse ECF/CCL11 in samples. No significant cross-reactivity or interference between Mouse ECF/CCL11 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-108 105
EDTA plasma (n=8) 92-105 98
Cell culture media (n=8) 92-103 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 167.16 438.75 1630.13 171.09 463.75 1723.54
Standard deviation 8.68 21.54 66.51 9.96 21.15 71.53
CV (%) 5.19 4.91 4.08 5.82 4.56 4.15
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance