Mouse CCL1 (Chemokine C-C-Motif Ligand 1) Sandwich ELISA (STJE0019701)

SKU:
STJE0019701

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.62-1000pg/mL
Short Description: This CCL1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ccl1
Gene ID: 20290
Uniprot ID: CCL1_MOUSE
Specificity: This kit recognizes Mouse CCL1 in samples. No significant cross-reactivity or interference between Mouse CCL1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name C-C Motif Chemokine 1
P500
Sis-Epsilon
Small-Inducible Cytokine A1
T-Cell Activation Protein 3
Tca-3
Tca3
Database Links Reactome: R-MMU-380108
Reactome: -MMU-418594
Alternative ELISA Names C-C Motif Chemokine 1 ELISA kit
P500 ELISA kit
Sis-Epsilon ELISA kit
Small-Inducible Cytokine A1 ELISA kit
T-Cell Activation Protein 3 ELISA kit
Tca-3 ELISA kit
Tca3 ELISA kit
Ccl1 ELISA kit
Scya1 ELISA kit
Tca3 ELISA kit
Specificity This kit recognizes Mouse CCL1 in samples. No significant cross-reactivity or interference between Mouse CCL1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-106 97
EDTA plasma (n=8) 86-101 93
Cell culture media (n=8) 85-96 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 48.70 145.48 429.84 48.66 140.94 401.59
Standard deviation 2.88 6.46 16.08 2.90 6.86 21.12
CV (%) 5.92 4.44 3.74 5.95 4.87 5.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance