| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
36.37pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This Bcl2L Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Bcl2l1 |
| Gene ID: |
12048 |
| Uniprot ID: |
B2CL1_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Widely expressed, with highest levels in the brain, thymus, bone marrow, and kidney. Bcl-X(L) and Bcl-X(delta-TM) expression is enhanced in B- and T-lymphocytes that have been activated. |
| Post Translational Modifications | Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome. |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis. |
| Protein Name | Bcl-2-Like Protein 1Bcl2-L-1Apoptosis Regulator Bcl-X |
| Database Links | Reactome: R-MMU-111453Reactome: -MMU-844455Reactome: -MMU-9648002 |
| Cellular Localisation | Mitochondrion MembraneSingle-Pass Membrane ProteinNucleus MembraneCytoplasmic SideCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes To The Centrosome When Phosphorylated At Ser-49Isoform Bcl-X(L): Mitochondrion Inner MembraneMitochondrion Outer MembraneMitochondrion MatrixCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneCytosolAfter Neuronal StimulationTranslocates From Cytosol To Synaptic Vesicle And Mitochondrion Membrane In A Calmodulin-Dependent MannerIsoform Bcl-X(Delta-Tm): Cytoplasm |
| Alternative ELISA Names | Bcl-2-Like Protein 1 ELISA kitBcl2-L-1 ELISA kitApoptosis Regulator Bcl-X ELISA kitBcl2l1 ELISA kitBcl2l ELISA kitBclx ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
