MAPK12 Positive Control for STJ501689 peptide (STJ505436)

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STJ505436-5

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Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: MAPK12 Positive Control for STJ501689 is synthetically produced from the sequence and is suitable for use in western blot applications.
Formulation: Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range: WB: 1:500
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: MAPK12
Gene ID: 6300
Uniprot ID: MK12_HUMAN
Specificity: This is positive control is recommended for use in combination with MAPK12 antibody STJ501689.
Tissue Specificity Highly expressed in skeletal muscle and heart.
Post Translational Modifications Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme. Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes.and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.
Peptide Name Mitogen-Activated Protein Kinase 12
Map Kinase 12
Mapk 12
Extracellular Signal-Regulated Kinase 6
Erk-6
Mitogen-Activated Protein Kinase P38 Gamma
Map Kinase P38 Gamma
Stress-Activated Protein Kinase 3
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-171007
Reactome: R-HSA-2151209
Reactome: R-HSA-376172
Reactome: R-HSA-4420097
Reactome: R-HSA-525793
Reactome: R-HSA-5675221
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Mitochondrial When Associated With Sh3bp5
In Skeletal Muscle Colocalizes With Snta1 At The Neuromuscular Junction And Throughout The Sarcolemma
Alternative Peptide Names Mitogen-Activated Protein Kinase 12 protein
Map Kinase 12 protein
Mapk 12 protein
Extracellular Signal-Regulated Kinase 6 protein
Erk-6 protein
Mitogen-Activated Protein Kinase P38 Gamma protein
Map Kinase P38 Gamma protein
Stress-Activated Protein Kinase 3 protein
MAPK12 protein
ERK6 protein
SAPK3 protein

Information sourced from Uniprot.org

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