| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.042ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This XPG Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
ERCC5 |
| Gene ID: |
2073 |
| Uniprot ID: |
ERCC5_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II. Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity. Required for DNA replication fork maintenance and preservation of genomic stability. Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site. During HRR, binds to the replication fork with high specificity and stabilizes it. Also, acts upstream of HRR, to promote the release of BRCA1 from DNA. |
| Protein Name | Dna Excision Repair Protein Ercc-5Dna Repair Protein Complementing Xp-G CellsXeroderma Pigmentosum Group G-Complementing Protein |
| Database Links | Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6782135 |
| Cellular Localisation | NucleusChromosomeColocalizes With Rad51 To Nuclear Foci In S PhaseLocalizes To Dna Double-Strand Breaks (Dbs) During Replication StressColocalizes With Brca2 To Nuclear Foci Following Dna Replication Stress |
| Alternative ELISA Names | Dna Excision Repair Protein Ercc-5 ELISA kitDna Repair Protein Complementing Xp-G Cells ELISA kitXeroderma Pigmentosum Group G-Complementing Protein ELISA kitERCC5 ELISA kitERCM2 ELISA kitXPG ELISA kitXPGC ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
