| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.056ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This XPD Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Dilution Range: | °C |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | ERCC2 |
| Gene ID: | 2068 |
| Uniprot ID: | ERCC2_HUMAN |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | ISGylated. |
| Function | ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase Subunit XpdTfiih Subunit XpdBasic Transcription Factor 2 80 Kda SubunitBtf2 P80CxpdDna Excision Repair Protein Ercc-2Dna Repair Protein Complementing Xp-D CellsTfiih Basal Transcription Factor Complex 80 Kda SubunitTfiih 80 Kda SubunitTfiih P80Xeroderma Pigmentosum Group D-Complementing Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-2564830Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle |
| Alternative ELISA Names | General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd ELISA kitTfiih Subunit Xpd ELISA kitBasic Transcription Factor 2 80 Kda Subunit ELISA kitBtf2 P80 ELISA kitCxpd ELISA kitDna Excision Repair Protein Ercc-2 ELISA kitDna Repair Protein Complementing Xp-D Cells ELISA kitTfiih Basal Transcription Factor Complex 80 Kda Subunit ELISA kitTfiih 80 Kda Subunit ELISA kitTfiih P80 ELISA kitXeroderma Pigmentosum Group D-Complementing Protein ELISA kitERCC2 ELISA kitXPD ELISA kitXPDC ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
