| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This XOD Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
XDH |
| Gene ID: |
7498 |
| Uniprot ID: |
XDH_HUMAN |
| Specificity: |
This kit recognizes Human XOD in samples. No significant cross-reactivity or interference between Human XOD and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Subject to partial proteolysis.this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro).this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). |
| Function | Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). |
| Protein Name | Xanthine Dehydrogenase/Oxidase Includes - Xanthine DehydrogenaseXd - Xanthine OxidaseXoXanthine OxidoreductaseXor |
| Database Links | Reactome: R-HSA-74259Reactome: R-HSA-8851680Reactome: R-HSA-9748787 |
| Cellular Localisation | CytoplasmPeroxisomeSecreted |
| Alternative ELISA Names | Xanthine Dehydrogenase/Oxidase Includes - Xanthine Dehydrogenase ELISA kitXd - Xanthine Oxidase ELISA kitXo ELISA kitXanthine Oxidoreductase ELISA kitXor ELISA kitXDH ELISA kitXDHA ELISA kit |
| Specificity | This kit recognizes Human XOD in samples. No significant cross-reactivity or interference between Human XOD and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-104 | 96 |
| EDTA plasma (n=8) | 94-106 | 100 |
| Cell culture media (n=8) | 94-106 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.47 | 0.85 | 4.85 | 0.49 | 0.79 | 4.64 |
| Standard deviation | 0.03 | 0.05 | 0.22 | 0.03 | 0.04 | 0.25 |
| CV (%) | 6.38 | 5.88 | 4.54 | 6.12 | 5.06 | 5.39 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
