| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human VEGFR-3/FLT-4-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FLT4 |
| Gene ID: |
2324 |
| Uniprot ID: |
VGFR3_HUMAN |
| Immunogen Region: |
Tyr25-Ile776 |
| Immunogen: |
Recombinant Human VEGFR-3/FLT-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr25-Ile776) of human VEGFR-3/FLT-4 (Accession #NP_002011.2) fused with 6×His tag at the C-terminus. |
| Post Translational Modifications | Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation in response to H(2)O(2) is mediated by a process that requires SRC and PRKCD activity. Phosphorylation at Tyr-1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at Tyr-1063 and Tyr-1337 is important for interaction with CRK and subsequent activation of MAPK8. Phosphorylation at Tyr-1230, Tyr-1231 and Tyr-1337 is important for interaction with GRB2 and subsequent activation of the AKT1 and MAPK1/ERK2 and/or MAPK3/ERK1 signaling pathways. In response to endothelial cell adhesion onto collagen, can also be phosphorylated in the absence of FLT4 kinase activity by SRC at Tyr-830, Tyr-833, Tyr-853, Tyr-1063, Tyr-1333, and Tyr-1337. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades.isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 3Vegfr-3Fms-Like Tyrosine Kinase 4Flt-4Tyrosine-Protein Kinase Receptor Flt4 |
| Database Links | Reactome: R-HSA-195399Reactome: R-HSA-9013695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmNucleusLigand-Mediated Autophosphorylation Leads To Rapid InternalizationIsoform 1: Cell MembraneIsoform 2: Cell MembraneIsoform 3: Secreted |
| Alternative Protein Names | Vascular Endothelial Growth Factor Receptor 3 proteinVegfr-3 proteinFms-Like Tyrosine Kinase 4 proteinFlt-4 proteinTyrosine-Protein Kinase Receptor Flt4 proteinFLT4 proteinVEGFR3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
