| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This VCAM-1/CD106 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
VCAM1 |
| Gene ID: |
7412 |
| Uniprot ID: |
VCAM1_HUMAN |
| Specificity: |
This kit recognizes Human VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human VCAM-1/CD106 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1V-Cam 1Vcam-1Incam-100Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-6785807Reactome: R-HSA-877300 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative ELISA Names | Vascular Cell Adhesion Protein 1 ELISA kitV-Cam 1 ELISA kitVcam-1 ELISA kitIncam-100 ELISA kitCd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 ELISA kitVCAM1 ELISA kit |
| Specificity | This kit recognizes Human VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human VCAM-1/CD106 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 83-98 | 90 |
| EDTA plasma (n=8) | 94-105 | 100 |
| Cell culture media (n=8) | 94-110 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.82 | 11.41 | 47.65 | 5.08 | 11.96 | 47.72 |
| Standard deviation | 0.27 | 0.50 | 2.48 | 0.28 | 0.50 | 2.07 |
| CV (%) | 5.60 | 4.38 | 5.20 | 5.51 | 4.18 | 4.34 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
