| Host: |
sf9 Insect cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Ubiquitin carboxyl-terminal hydrolase 16-His Tag protein was developed from sf9 insect cells and has a target region of His Tag. For use in research applications. |
| Formulation: |
20 mM Tris, 150 mM NaCl, 2 mM BetaME, 10% Glycerol |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Post Translational Modifications | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
| Function | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A: deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 16Deubiquitinating Enzyme 16Ubiquitin Thioesterase 16Ubiquitin-Processing Protease Ubp-MUbiquitin-Specific-Processing Protease 16 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Protein Names | Ubiquitin Carboxyl-Terminal Hydrolase 16 proteinDeubiquitinating Enzyme 16 proteinUbiquitin Thioesterase 16 proteinUbiquitin-Processing Protease Ubp-M proteinUbiquitin-Specific-Processing Protease 16 proteinUSP16 proteinMSTP039 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
