| Host: |
E. coli |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human UBE2D3-C-His protein was developed from e. coli and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Gene Symbol: |
UBE2D3 |
| Gene ID: |
7323 |
| Uniprot ID: |
UB2D3_HUMAN |
| Immunogen Region: |
Met1-Met147 |
| Immunogen: |
Recombinant Human UBE2D3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of human UBE2D3 (Accession #NP_003331.1) fused with a 6×His tag at the C-terminus. |
| Post Translational Modifications | Phosphorylated by AURKB. |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction. Together with RNF135, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. Together with ZNF598, catalyzes ubiquitination of 40S ribosomal proteins in response to ribosome collisions. In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 D3(E3-Independent E2 Ubiquitin-Conjugating Enzyme D3E2 Ubiquitin-Conjugating Enzyme D3Ubiquitin Carrier Protein D3Ubiquitin-Conjugating Enzyme E2(17kb 3Ubiquitin-Conjugating Enzyme E2-17 Kda 3Ubiquitin-Protein Ligase D3 |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-168927Reactome: R-HSA-201451Reactome: R-HSA-2173795Reactome: R-HSA-5357905Reactome: R-HSA-8866654Reactome: R-HSA-8951664Reactome: R-HSA-9033241Reactome: R-HSA-936440Reactome: R-HSA-937041Reactome: R-HSA-9705462Reactome: R-HSA-983168 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinEndosome Membrane |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 D3 protein(E3-Independent E2 Ubiquitin-Conjugating Enzyme D3 proteinE2 Ubiquitin-Conjugating Enzyme D3 proteinUbiquitin Carrier Protein D3 proteinUbiquitin-Conjugating Enzyme E2(17kb 3 proteinUbiquitin-Conjugating Enzyme E2-17 Kda 3 proteinUbiquitin-Protein Ligase D3 proteinUBE2D3 proteinUBC5C proteinUBCH5C protein |
Information sourced from Uniprot.org
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