| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human UBA52 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
UBA52 |
| Gene ID: |
7311 |
| Uniprot ID: |
RL40_HUMAN |
| Specificity: |
This kit recognizes Human UBA52 in samples. No significant cross-reactivity or interference between Human UBA52 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30. Ubiquitin: Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones. Ubiquitin: (Microbial infection) Mono-ADP-ribosylated at Thr-66 by the C.violaceum CteC virulence factor. ADP-ribosylation causes the shutdown of polyubiquitin synthesis and disrupts the recognition and reversal of polyubiquitin. |
| Function | Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair.Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation.Lys-29-linked is involved in proteotoxic stress response and cell cycle.Lys-33-linked is involved in kinase modification.Lys-48-linked is involved in protein degradation via the proteasome.Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Large ribosomal subunit protein eL40: Component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs. |
| Protein Name | Ubiquitin-Ribosomal Protein El40 Fusion ProteinCep52Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 Cleaved Into - Ubiquitin - Large Ribosomal Subunit Protein El4060s Ribosomal Protein L40 |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-1169091Reactome: R-HSA-1169408Reactome: R-HSA-1234176Reactome: R-HSA-1236382Reactome: R-HSA-1236974Reactome: R-HSA-1253288Reactome: R-HSA-1295596Reactome: R-HSA-1358803Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-162588Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-168928Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174113Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-174490Reactome: R-HSA-175474Reactome: R-HSA-179409Reactome: R-HSA-1799339Reactome: R-HSA-180534Reactome: R-HSA-180585Reactome: R-HSA-182971Reactome: R-HSA-187577Reactome: R-HSA-192823Reactome: R-HSA-195253Reactome: R-HSA-201681Reactome: R-HSA-202424Reactome: R-HSA-205043Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-211733Reactome: R-HSA-2122947Reactome: R-HSA-2122948Reactome: R-HSA-2173788Reactome: R-HSA-2173791Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-2408557Reactome: R-HSA-2467813Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559585Reactome: R-HSA-2565942Reactome: R-HSA-2644606Reactome: R-HSA-2672351Reactome: R-HSA-2691232Reactome: R-HSA-2871837Reactome: R-HSA-2894862Reactome: R-HSA-2979096Reactome: R-HSA-3134975Reactome: R-HSA-3322077Reactome: R-HSA-349425Reactome: R-HSA-3769402Reactome: R-HSA-3785653Reactome: R-HSA-382556Reactome: R-HSA-400253Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-450408Reactome: R-HSA-4608870Reactome: R-HSA-4641257Reactome: R-HSA-4641258Reactome: R-HSA-4641263Reactome: R-HSA-5205685Reactome: R-HSA-532668Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5358346Reactome: R-HSA-5362768Reactome: R-HSA-5607761Reactome: R-HSA-5607764Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5632684Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5658442Reactome: R-HSA-5668541Reactome: R-HSA-5675221Reactome: R-HSA-5675482Reactome: R-HSA-5676590Reactome: R-HSA-5678895Reactome: R-HSA-5684264Reactome: R-HSA-5685942Reactome: R-HSA-5687128Reactome: R-HSA-5689603Reactome: R-HSA-5689877Reactome: R-HSA-5689880Reactome: R-HSA-5689896Reactome: R-HSA-5689901Reactome: R-HSA-5693565Reactome: R-HSA-5693607Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6783310Reactome: R-HSA-6791226Reactome: R-HSA-6804756Reactome: R-HSA-6804757Reactome: R-HSA-6804760Reactome: R-HSA-6807004Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-69017Reactome: R-HSA-69231Reactome: R-HSA-69481Reactome: R-HSA-69541Reactome: R-HSA-69601Reactome: R-HSA-72689Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-75815Reactome: R-HSA-8849469Reactome: R-HSA-8852276Reactome: R-HSA-8854050Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8863795Reactome: R-HSA-8866427Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-8875360Reactome: R-HSA-8876493Reactome: R-HSA-8939236Reactome: R-HSA-8939902Reactome: R-HSA-8941858Reactome: R-HSA-8948747Reactome: R-HSA-8948751Reactome: R-HSA-8951664Reactome: R-HSA-901032Reactome: R-HSA-9010553Reactome: R-HSA-9013507Reactome: R-HSA-9013973Reactome: R-HSA-9014325Reactome: R-HSA-9020702Reactome: R-HSA-9033241Reactome: R-HSA-909733Reactome: R-HSA-912631Reactome: R-HSA-917729Reactome: R-HSA-917937Reactome: R-HSA-936440Reactome: R-HSA-936964Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-937042Reactome: R-HSA-937072Reactome: R-HSA-9604323Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9633012Reactome: R-HSA-9636383Reactome: R-HSA-9637628Reactome: R-HSA-9645460Reactome: R-HSA-9646399Reactome: R-HSA-9648002Reactome: R-HSA-9664873Reactome: R-HSA-9680350Reactome: R-HSA-9683683Reactome: R-HSA-9692916Reactome: R-HSA-9694493Reactome: R-HSA-9705462Reactome: R-HSA-9705671Reactome: R-HSA-9706369Reactome: R-HSA-9706377Reactome: R-HSA-9708530Reactome: R-HSA-975110Reactome: R-HSA-975144Reactome: R-HSA-975163Reactome: R-HSA-9755511Reactome: R-HSA-9758274Reactome: R-HSA-975956Reactome: R-HSA-975957Reactome: R-HSA-9762114Reactome: R-HSA-977225Reactome: R-HSA-983168 |
| Cellular Localisation | Ubiquitin: CytoplasmNucleusLarge Ribosomal Subunit Protein El40: Cytoplasm |
| Alternative CLIA Names | Ubiquitin-Ribosomal Protein El40 Fusion Protein CLIA kitCep52 CLIA kitUbiquitin A-52 Residue Ribosomal Protein Fusion Product 1 Cleaved Into - Ubiquitin - Large Ribosomal Subunit Protein El40 CLIA kit60s Ribosomal Protein L40 CLIA kitUBA52 CLIA kitUBCEP2 CLIA kit |
| Specificity | This kit recognizes Human UBA52 in samples. No significant cross-reactivity or interference between Human UBA52 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-109 | 104 |
| EDTA plasma(n=8) | 99-116 | 106 |
| Cell culture media(n=8) | 97-112 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 206.43 | 617.17 | 1877.26 | 190.28 | 606.66 | 2035.73 |
| Standard deviation | 24.07 | 61.41 | 121.08 | 20.30 | 69.64 | 216.40 |
| CV (%) | 11.66 | 9.95 | 6.45 | 10.67 | 11.48 | 10.63 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
