| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human TP63 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
TP63 |
| Gene ID: |
8626 |
| Uniprot ID: |
P63_HUMAN |
| Specificity: |
This kit recognizes Human TP63 in samples. No significant cross-reactivity or interference between Human TP63 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. |
| Post Translational Modifications | May be sumoylated. Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. |
| Function | Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. |
| Protein Name | Tumor Protein 63P63Chronic Ulcerative Stomatitis ProteinCuspKeratinocyte Transcription Factor KetTransformation-Related Protein 63Tp63Tumor Protein P73-LikeP73lP40P51 |
| Database Links | Reactome: R-HSA-139915Reactome: R-HSA-5620971Reactome: R-HSA-5628897Reactome: R-HSA-6803204Reactome: R-HSA-6803205Reactome: R-HSA-6803207Reactome: R-HSA-6803211Reactome: R-HSA-6804759 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Tumor Protein 63 CLIA kitP63 CLIA kitChronic Ulcerative Stomatitis Protein CLIA kitCusp CLIA kitKeratinocyte Transcription Factor Ket CLIA kitTransformation-Related Protein 63 CLIA kitTp63 CLIA kitTumor Protein P73-Like CLIA kitP73l CLIA kitP40 CLIA kitP51 CLIA kitTP63 CLIA kitKET CLIA kitP63 CLIA kitP73H CLIA kitP73L CLIA kitTP73L CLIA kit |
| Specificity | This kit recognizes Human TP63 in samples. No significant cross-reactivity or interference between Human TP63 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 97 |
| EDTA plasma(n=8) | 88-104 | 95 |
| Cell culture media(n=8) | 101-113 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 96.88 | 281.05 | 954.41 | 97.33 | 298.16 | 1028.76 |
| Standard deviation | 8.74 | 20.71 | 83.03 | 9.84 | 21.86 | 64.40 |
| CV (%) | 9.02 | 7.37 | 8.70 | 10.11 | 7.33 | 6.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
