• Human TNNI3 (Troponin I, cardiac muscle) Sandwich ELISA (STJE0002373)

Human TNNI3 (Troponin I, cardiac muscle) Sandwich ELISA (STJE0002373)

SKU:
STJE0002373

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.23ng/mL
Detection Limit: 0.39~25ng/mL
Short Description: This TNNI3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNNI3
Gene ID: 7137
Uniprot ID: TNNI3_HUMAN
Specificity: This kit recognizes Human TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Human TNNI3/cTn-I and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Name Troponin I - Cardiac Muscle
Cardiac Troponin I
Database Links Reactome: R-HSA-390522
Reactome: R-HSA-5578775
Alternative ELISA Names Troponin I - Cardiac Muscle ELISA kit
Cardiac Troponin I ELISA kit
TNNI3 ELISA kit
TNNC1 ELISA kit
Specificity This kit recognizes Human TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Human TNNI3/cTn-I and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-101 96
EDTA plasma (n=8) 96-111 102
Cell culture media (n=8) 91-105 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.20 3.00 8.70 1.30 2.70 8.50
Standard deviation 0.10 0.20 0.40 0.10 0.10 0.40
CV (%) 8.33 6.67 4.60 7.69 3.70 4.71
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance