Name: Human TNFSF5/CD40 ligand/CD154 protein (Recombinant) (C-His) (STJP002929)
SKU: STJP002929
Availability: InStock

General Information
Product Properties
Target Information
Additional Info
Product Review

Host:	HEK293 cells
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Formulation:	Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method:	< 0.1 EU/Mu g
Immunoreactivity:	Measured by its binding ability in a functional ELISA.Immobilized Human CD40L at 5 Mu g/mL (100 Mu L/well) can bind Human CD40 with a linear range of 0.005-0.5 Mu g/mL.

Gene Symbol:	CD40LG
Gene ID:	959
Uniprot ID:	CD40L_HUMAN
Immunogen Region:	Gly116-Leu261
Immunogen:	Active Recombinant Human CD40-L/CD40L/TNFSF5/TRAP/CD40LG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly116-Leu261) of human CD40-L/CD40L/TNFSF5/TRAP/CD40LG (Accession #NP_000065.1) fused with
Immunogen Sequence:	CDLPQTHSLGNRRALILLAQ MGRISPFSCLKDRHDFGLPQ EEFDGNQFQKTQAISVLHEM IQQTFNLFSTEDSSAAWEQS LLEKFSTELYQQLNNLEACV IQEVGMEETPLMNEDSILAV RKYFQRITLYLTEKKYSPCA WEVVRAEIMRSLSFSTNLQK ILRRKD

Tissue Specificity	Specifically expressed on activated CD4+ T-lymphocytes.
Post Translational Modifications	The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated.
Function	Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3.both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Protein Name	Cd40 Ligand Cd40-L T-Cell Antigen Gp39 Tnf-Related Activation Protein Trap Tumor Necrosis Factor Ligand Superfamily Member 5 Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form Scd40l
Database Links	Reactome: R-HSA-198933 Reactome: R-HSA-5668541 Reactome: R-HSA-5676594
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Cell Surface Cd40 Ligand Soluble Form: Secreted Release Of Soluble Cd40l From Platelets Is Partially Regulated By Gp Iib/Iiia Actin Polymerization And A Matrix Metalloproteinases (Mmp) Inhibitor-Sensitive Pathway
Alternative Protein Names	Cd40 Ligand protein Cd40-L protein T-Cell Antigen Gp39 protein Tnf-Related Activation Protein protein Trap protein Tumor Necrosis Factor Ligand Superfamily Member 5 protein Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form protein Scd40l protein CD40LG protein CD40L protein TNFSF5 protein TRAP protein

Information sourced from Uniprot.org

Model	Name	Host
STJP003038	Mouse TNFSF5/CD40 ligand/CD154 protein (Recombinant) (C-His) (STJP003038)	HEK293 cells
STJP002733	Human TNFRSF5/CD40 protein (Recombinant) (C-His) (STJP002733)	HEK293 cells
STJP001233	Human TNFRSF5/CD40 protein (Recombinant) (C-hFc&His) (STJP001233)	HEK293 cells
STJP002723	Human Flt4 ligand/VEGF-C protein (Recombinant) (C-His) (STJP002723)	HEK293 cells
STJP001425	Human TNFSF18/GITR Ligand protein (Recombinant) (C-His) (STJP001425)	HEK293 cells

Human TNFSF5/CD40 ligand/CD154 protein (Recombinant) (C-His) (STJP002929)

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